Composite

Part:BBa_K360142:Design

Designed by: Jorge Eduardo Buendía Buendía   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-22)


Strong RBS with spacer + GFP BBa_E0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 661


Design Notes

7 bp where added to RBS as spacer.


Source

The Strong RBS and spacer where taken from lacZ gene of Escherichia coli. The GFP BBa_E0040 was taken from the already registered part.

References